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Gilson Pipetman (formerly Pipetman G) and Pipetman L – Preventative Maintenance

A triangular warning sign with a red border and a black exclamation mark inside a white center, indicating caution or attention—similar to the care needed when handling a Gilson Microman.

The process of performing preventative maintenance for the Gilson Pipetman (formerly Pipetman G) and Pipetman L requires great care, a high attention to detail, patience, and steady hands. Please dedicate a good work area with good lighting and space where small parts can be located should they pop out during disassembly. Pipette damage and lost parts may occur if attention to detail is not maintained. Please proceed at your own risk.

Replacement Parts:

  1. Pipetman G/L O-rings
  2. Seals & Seal Guides
  3. Gilson Pipetman G/L Grease

The 2µL, 5mL, and 10mL models use the same seal and o-ring as the Pipetman Classic and Neo. Additionally, the Pipetman single channel has now replaced the Pipetman G. The new model uses the same parts as the Pipetman G.

Recommend Supplies:

  1. Gloves
  2. Cotton Swabs
  3. Lint Free Wipe
  4. Isopropanol (rubbing alcohol)
Step 1:

Press down the ejector button to expose the ejector clip.

A gloved hand presses down the plunger of a Pipetman pipette, indicated by a red downward arrow.
Step 2:

While holding the ejector button down, turn the tip ejector counterclockwise to unclip it.

Removing pipetman ejector
Step 3:

Turn the connecting nut counterclockwise to remove it from the pipette body.

A gloved hand removes the connecting nut of a Pipetman pipette, with a red arrow indicating the direction to turn the nut.
Step 4:

Remove the piston assembly from the tip holder.

Removing the piston assembly from the Pipetman tip holder.
Step 5:

Inspect the tip holder for any debris or seals/o-rings.

Step 6:

For 10µL to 1000µL models:
Remove the seal guide and o-ring (or the inner and outer o-rings on the 1000 µL model) from the piston.

Top: Gloved hand holding a small Pipetman piston. Bottom: Close-up of a Pipetman 1000µL seal holder with a yellow crown piece and two black o-rings, both highlighted with red circles.

For 2µL, 5mL, and 10mL models:
Remove the seal and o-ring from the piston.

Step 7:

Clean the piston assembly using a lint free wipe moistened with isopropanol. Allow it to dry, then apply Gilson Pipetman G/L Grease.

A gloved hand holds a piece of gauze, cleaning or wiping a Pipetman piston against a plain background.
A gloved hand greasing a Pipetman piston
Step 8:

For 10µL to 1000µL models:
Install the seal guide and new o-ring (or inner and outer o-rings on the 1000µL model) onto the piston.

A gloved hand holds a Pipetman pipette piston, showing the seal guide and o-rings for different sizes, labeled with red circles for assembly reference.


For 2µL, 5mL, and 10mL models:
Install the new seal and o-ring onto the piston.

Step 9:

Reinsert the piston assembly into the tip holder and push it upward toward the pipette body.

A gloved hand holds a Pipetman piston assembly, aligning a metal spring with a tip holder against a plain background.

For 5mL and 10mL models: Thread the tip cone onto the piston assembly.

Step 10:

Turn the connecting nut clockwise to secure it to the pipette body.

A gloved hand twists the connecting nut onto a pipette, with a red arrow indicating the direction to turn.

Note: For 5mL and 10mL models, the piston assembly will thread directly into the pipette body.

Step 11:

While holding the ejector button down, turn the tip ejector clockwise to clip it into the ejector assembly.

A person wearing blue gloves reattaches the tip ejector to the ejector assembly; a red arrow indicates the direction of rotation.

Related Posts:
Gilson Pipetman Neo, G, & L Multichannel – Lower Assembly Disassembly
Gilson Pipetman L – Counter Assembly Replacement
Pipetman L Ejector Maintenance
Gilson Pipetman L Calibration Tool, Item # 28888

Disclaimer: Any action you take using the information on this website is strictly at your own risk. The information herein does not constitute professional advice and is general in nature. We make no warranty that this information will meet your requirements, be safe, accurate, or error-free. Pipette Supplies, Inc. is not responsible for any errors or omissions, any results obtained from the use of this information, or any loss or damage arising out of the use of this information. This site is for educational purposes only.
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