Gilson Pipetman L Calibration Tool, Item # 28888

Instructions to use the Pipetman L Calibration Tool. Link to product page: 28888

This tool is for increasing or decreasing the pick-up volume during the calibration process.  Review and follow the Gilson publication, Verification Procedure for Accuracy and Precision.  Contact Us for a copy.

Be sure that the sealing system of the pipette (piston, tip holder, seal and o-ring) is functioning properly.  The pipette liquid end must be leak-free.  If it is not, calibration will not be effective. 

Printable Calibration Log
Printable Calibration Stickers (for use with Avery 5960 labels)

CALIBRATION INSTRUCTIONS:

Step 1:

Set the Pipetman L pipette to its minimum test volume according to the user manual (usually 10% of the nominal volume). 

Step 2:

Pre-rinse the tip. Dispense and weigh four individual samples. Determine the mean weight of the samples in micro-liters (µl). If the mean is not within accuracy limits, continue with the calibration.

Step 3:

Remove the plunger button from the pipette by pulling it straight up.  Push the volume control disc down to the lock position.

Step 4:

Insert the two prongs, C, of the Pipetman L calibration tool into the two slots of the center access button of the volume control disc. Rotate the tool counterclockwise about 15 degrees and pull out. The center access button will come out loaded on to the two prongs. Hold the button in one hand and rotate the tool in clockwise direction to disengage and unload the button from the tool.

Step 5:

Holding on to the large knob A and letting the outer barrel with the knob B slip down on its own weight, slide the tool over the plunger rod and let it go inside the pipette body as far as it can on its own weight.

Step 6:

While pressing down VERY slightly, rotate the knob B.  The two prongs of the knob B will engage with the square part of the calibration mechanism of the pipette in less than 45 degree of rotation.  

Step 7:

Rotate the large knob A without pressing down.  The groove C of the inner rod will fit over the inner member of the calibration mechanism.

Step 8:

To increase the pick-up volume, hold the knurled knob B firmly without letting it move and rotate the large knob A in counter clock direction; to decrease the pick-up volume rotate the knob A in clockwise direction.  Rotate ONLY about half turn at a time and re-check the mean weight as done in step 2.

NOTE:  You do not need to put back the center access button into the volume control disc at this stage, just replace the plunger button making sure that the flats of the plunger button align with the flats of the plunger rod.

Step 9:

Check the pipette at the 10% volume as done in step 2.  If the pipette is within the specification, continue with the next step.  If not, return to step 2.

Step 10:

Set the pipette to its maximum volume and perform the calibration test.  If the instrument is within accuracy limits, it has been calibrated successfully.  If it is outside the accuracy limits, return to step 2 and repeat the process.

Step 11:

Remove the plunger button again.  Load the center access button on to the two prongs C of the Pipetman L calibration tool, insert the button in its place and turn the tool about 15 degrees clockwise. The button will lock in the volume control disc.  Pull out the tool and install the plunger button on the plunger rod.

“Pipetman” is a registered trademark of Gilson, S.A.S.

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