Pipetman Calibration Wrench, Item # 27777

Pipetman Calibration Instructions:

Printable Calibration Log
Printable Calibration Stickers (for use with Avery 5960 labels)

Link to product page: 27777

This wrench is used for increasing or decreasing the pick-up volume during the calibration process on Pipetman versions that have 6 holes on the top of the Calibration Screw. 

Review and follow the Gilson publication, Verification Procedure for Accuracy and Precision. Contact Us for a copy.

Be sure that the sealing system of the pipette (piston, tip holder, seal and o-ring) is functioning properly.  The pipette liquid end must be leak-free.  If it is not, calibration will not be effective. 

CALIBRATION:

Step 1:

First, set the pipette to its minimum test volume according to the user’s manual (usually 10% of the nominal volume). 

Step 2:

Pre-rinse the tip, dispense and weigh four individual samples. Determine the mean weight of the samples in micro-liters (µl). If the mean is not within accuracy limits, continue with the calibration.

Step 3:

Hold the Volume Adjustment Knob to prevent it from turning.  Place the 5 prongs of the wrench into 5 of the 6 holes on the top of the Calibration Screw, which is located inside the Volume Adjustment Knob.

Step 4:

To reduce pickup volume:  Turn slightly clockwise.
To increase pickup volume:  Turn slightly counterclockwise.

Step 5:

Remove the calibration wrench. 

Step 6:

After completing these adjustments, check the instrument at 10% of nominal volume as described in step 1. If the pipette is within specification at 10% nominal value, continue to the next step. If not, return to step 1 and perform the procedure again until the instrument is within specification limits.

Step 7:

Lastly, set the pipette to its maximum volume and then perform the calibration test. If the instrument is within accuracy limits, then it has been calibrated successfully. If it is outside of the accuracy limits, return to step 1 and repeat the process.

“Pipetman” is a registered trademark of Gilson, S.A.S.

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