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How to Calibrate an Eppendorf Research Plus Fixed Pipette

Instructions to Calibrate an Eppendorf Research-Plus Fixed Volume Pipette:

Printable Calibration Log
Printable Calibration Stickers (for use with Avery 5960 labels)

Step 1
  • Remove:
    • The ejector button (A) by pushing firmly upward to remove it from the pipette body.
    • The cover panel (B) by pushing in on the left side and then pushing up from the bottom colored-band portion.
    • The gray colored ring (C) and the colored plunger button cap (D) by firmly pulling upward.
Research Plus ejector system disassembled and labeled
Step 2
  • With a pair or rubber tipped pliers, grab and hold the brass-colored plunger rod at the location shown (E) to prevent it from rotating when the plunger button needs to be rotated and removed
  • With part (E) secure and not rotating, gently turn, rotate, and unthread the plunger button from the threaded portion of the brass-colored plunger rod (F).
Calibration screw
Step 3
  • To calibrate, place the Research Plus Fixed adjustment tool (EP 3120802002) over the calibration screw. Make very subtle turns of counter-clockwise to increase & clockwise to reduce desired volume.
  • Remove the calibration adjustment tool and check the volume aspirated/dispensed on a balance. Replace all removed parts after desired calibration results are achieved.
Calibration adjustment tool inserted into pipette

Disclaimer: Any action you take using the information on this website is strictly at your own risk. The information herein does not constitute professional advice and is general in nature. We make no warranty that this information will meet your requirements, be safe, accurate, or error-free. Pipette Supplies, Inc. is not responsible for any errors or omissions, any results obtained from the use of this information, or any loss or damage arising out of the use of this information. This site is for educational purposes only.
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