How to Adjust the Calibration for a Sartorius mLINE Single Channel Pipette

mLINE Calibration Instructions:

ATTENTION: Performing a calibration adjustment on any pipette should always be the last option, never the first. Performing a calibration adjustment first may mask underlying pipette performance issues that could affect both the short- and long-term health of your pipette. This could lead to costly repairs and part(s) replacement, and possibly the need to replace the pipette itself.  Adjusting the pipette calibration should only occur after the pipette sealing system, (i.e.: piston, shaft, o-ring, lubricant, pipette tips) and other critical components are inspected to ensure good working condition. If not replace part(s) as needed first. If you are unsure if the parts are in good condition or which parts should be replaced, please contact Pipettesupplies.com.

Printable Calibration Log
Printable Calibration Stickers (for use with Avery 5960 labels)

Recommended mLine Pipette Calibration Adjustment Tools and Needed Equipment:

• mLine Calibration Tool (Pipette Supplies Part Number SA 726203)
• Pipette Tips (for checking the volume delivered after calibration adjustment is made)
• Di Water non-aerated (for verifying the accuracy/precision after a calibration adjustment)
• 4-5 Place Balance (to gravimetrically confirm the desired volume aspirated and delivered is accurate and precise.)

Calibration Adjustment Process:

Step 1: Insert part B of the calibration tool into the calibration cap cover (SA 726006) that is located on the backside of the pipette body and gently pry off and remove the calibration cap. Lay aside in a safe location for re-installation later on. The calibration screw is now visible.

Step 2: (NOTE) Notice that the calibration screw will have a + and – symbol etched on it to indicate which direction the calibration adjustment tool should be turned to either increase (+) or decrease (-) the desired volume aspirated and dispensed by the pipette.

Step 3: (NOTE) If the current actual volume is lower or higher than desired then insert calibration tool:

Lower than desired: Slowly rotate the calibration adjustment tool no more than 1/8 of a turn in the counter–clockwise direction. This will increase the volume of liquid aspirated.

Higher than desired: Slowly rotate the calibration adjustment tool no more than 1/8 of a turn in the clockwise direction. This will decrease the volume of liquid aspirated.

Important: It is strongly recommended that any calibration adjustments occur at the 10% volume. This helps to ensure accuracy and precision is maintained throughout the pipette volume range (i.e., 20uL-200uL, 100uL -1000uL, etc.). Failure to do this could skew or affect the volume aspirated and dispensed at different tested volume points.

After Calibration Adjustment Process:

• Remove the calibration adjustment tool from the pipette calibration screw
• Install a pipette tip onto the pipette shaft
• Pre-rinse the tip 2-3 times with Di-water
• Aspirate and dispense the di water into a reservoir on a 4-5 place balance to confirm the new volume that is now being delivered
• Repeat the calibration adjustment process if the new volume delivered is still too low or too high

(NOTE) If the pipette is not delivering the desired volume after performing calibration adjustments for 2-3 minutes, lay the pipette aside and allow it to equilibrate back to ambient lab conditions for 60 minutes. After this time period lapses, re-check the pipette calibration (i.e., due to possible hand warming effects).

Step 4: After the desired volume is verified on a 4-5 place balance, re-install the calibration cap. The mLINE pipette is now ready for use.